人肝活检组织中的酶活性：对照组织和血清中某些溶酶体酶和膜结合酶的测定方法及活性
Enzyme activities in human liver biopsies: assay methods and activities of some lysosomal and membrane-bound enzymes in control tissue and serum.
作者信息
Seymour C A, Peters T J
出版信息
Clin Sci Mol Med. 1977 Mar;52(3):229-39. doi: 10.1042/cs0520229.
PMID:14804
Abstract
- Highly sensitive technique are described for the assay of plasma membrane (5'-nucleotidase, alkaline phosphatase), microsomal (neutral alpha-glucosidase, leucyl-2-naphthylamidase) and biliary canalicular (gamma-glutamyltransferase) enzymes and for nine acid hydrolases (acid phosphatase, phosphodiesterase, beta-glucosidase, alpha-glucosidase, alpha-galactosidase, beta-galactosidase, alpha-mannosidase, N-acetyl-beta-glucosaminidase, beta-glucuronidase) in human liver. 2. Optimum and specific assay systems have been developed which give linear kinetics for all enzymes. 3. The range of enzyme activities in samples of human liver, obtained by closed needle biopsy, and sera have been determined.
摘要
- 本文描述了用于检测人肝脏中质膜（5'-核苷酸酶、碱性磷酸酶）、微粒体（中性α-葡萄糖苷酶、亮氨酰-2-萘胺酶）和胆小管（γ-谷氨酰转移酶）酶以及九种酸性水解酶（酸性磷酸酶、磷酸二酯酶、β-葡萄糖苷酶、α-葡萄糖苷酶、α-半乳糖苷酶、β-半乳糖苷酶、α-甘露糖苷酶、N-乙酰-β-葡萄糖胺酶、β-葡萄糖醛酸酶）的高灵敏度技术。2. 已开发出最佳且特异的检测系统，该系统能为所有酶提供线性动力学。3. 已确定通过闭针活检获得的人肝脏样本和血清中的酶活性范围。
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