Pástorová B, Sova O, Bruda J
Physiol Bohemoslov. 1978;27(1):69-73.
The authors studied the effect of a protein-free diet on 14C-thymidine incorporation into rat liver DNA in vivo and found, after 2-3 weeks, a marked decrease in uptake of the radioactive base into the liver DNA, followed by a decrease in the proportion of DNA in liver cell homogenates. The total nuclear count/mg liver tissue displayed an increase during protein depletion, except for the 5th week, when a decrease was recorded. The incorporation of 14C thymidine into the brain DNA likewise displayed no great differences, although a significant drop was observed during the 2nd to 4th week of depletion. In the 5th week we recorded an increase in uptake of the radioactive base by brain DNA, exceeding the incorporation values in the controls.
作者研究了无蛋白饮食对14C-胸苷在体内掺入大鼠肝脏DNA的影响，发现2至3周后，放射性碱基掺入肝脏DNA的量显著减少，随后肝细胞匀浆中DNA的比例降低。除第5周记录到减少外，每毫克肝脏组织的细胞核总数在蛋白质缺乏期间呈增加趋势。14C胸苷掺入脑DNA的情况同样没有很大差异，尽管在缺乏的第2至4周观察到显著下降。在第5周，我们记录到脑DNA对放射性碱基的摄取增加，超过了对照组的掺入值。
