Polotskiĭ Iu E, Efremov V E, Bondarenko V M, Kuz'min V A, Smirnova G V, Romanova Iu M, Belov A G, Maksimov P N
Zh Mikrobiol Epidemiol Immunobiol. 1992(9-10):50-7.
Chickens over 10 days old, infected orally with virulent salmonellae, were found to remain alive. Histologic investigation showed the development of mild enteritis and more pronounced, lasting for more than two weeks, inflammation of the cecum, dissemination and focal lesions in the liver (granulomas, necrosis). In experiments on the oral immunization of 3-day old chickens the bivalent hybrid of S. typhimurium vaccine strain 274 and S. dublin induced only pronounced blast transformation in lymphatic follicles of the cecum, hyperplasia of activated macrophages and formation of granulomas from these macrophages and lymphocytes. After oral challenge of the immunized chickens with virulent salmonellae of group B (S. typhimurium) and group D (S. enteritidis, S. gallinarum-pullorum) the chickens exhibited sharply pronounced protection against adhesion, colonization and invasion, and a few penetrating bacteria were rapidly destroyed by immune macrophages. Hybrid strain 274/O9 proved to be suitable for use as oral bivalent vaccine against salmonellosis in chickens.
10日龄以上的鸡经口感染强毒沙门氏菌后仍存活。组织学研究显示，出现了轻度肠炎，盲肠炎症更明显且持续两周以上，肝脏有扩散性和局灶性病变（肉芽肿、坏死）。在对3日龄鸡进行口服免疫的实验中，鼠伤寒沙门氏菌疫苗株274和都柏林沙门氏菌的二价杂交株仅在盲肠淋巴滤泡中诱导出明显的母细胞转化、活化巨噬细胞增生以及由这些巨噬细胞和淋巴细胞形成肉芽肿。用B组（鼠伤寒沙门氏菌）和D组（肠炎沙门氏菌、鸡白痢沙门氏菌）的强毒沙门氏菌对免疫后的鸡进行口服攻毒后，鸡对黏附、定植和侵袭表现出显著的保护作用，少数侵入的细菌会被免疫巨噬细胞迅速消灭。杂交株274/O9被证明适合用作鸡沙门氏菌病的口服二价疫苗。
