Batellier J, Wassef M, Merval R, Duriez M, Tedgui A
INSERM U 141, Hôpital Lariboisière, Paris.
Arch Mal Coeur Vaiss. 1992 Aug;85(8):1213-7.
Vein grafts undergo early intimal thickening and accelerated atherosclerosis. To assess the role of increased wall stress and distension in the pathogenic responses, 11 New Zealand white rabbits underwent interposition of an autologous jugular vein graft in the left common carotid artery. To relieve wall stress and reduce distension, the half proximal part of the vein was wrapped with a polytetrafluoroethylene graft (i.d. 4 mm). Animals were fed 1% cholesterol for 8 weeks. Vein graft and carotid artery were perfusion fixed with Karnovsky solution at 100 mmHg. They were stained with Sudan IV, and 5-microns cross sections were stained with hematoxylin-eosin and orcein. The internal diameter was reduced by 46 +/- 10% in wrapped vein graft segments as compared with unwrapped ones. The percentage of luminal surface covered by sudanophilic lesions (%AS) was assessed by automatic planimetry. Results (mean +/- SD) were as follows. [table: see text]. Abundant foam cells were found in the intima of unwrapped veins, whereas they were absent or rare in wrapped segments. We concluded that atherosclerotic lesions could be prevented in vein grafts by reducing wall stress and distension.
静脉移植物会经历早期内膜增厚和动脉粥样硬化加速。为了评估壁应力增加和扩张在致病反应中的作用，11只新西兰白兔接受了在左颈总动脉中植入自体颈静脉移植物的手术。为了减轻壁应力并减少扩张，静脉的近端一半用聚四氟乙烯移植物（内径4毫米）包裹。给动物喂食1%的胆固醇，持续8周。静脉移植物和颈动脉在100 mmHg压力下用卡诺夫斯基溶液进行灌注固定。它们用苏丹IV染色，5微米厚的横截面用苏木精-伊红和地衣红染色。与未包裹的静脉移植物节段相比，包裹的静脉移植物节段的内径减少了46±10%。通过自动平面测量法评估嗜苏丹病变覆盖的管腔表面百分比（%AS）。结果（平均值±标准差）如下。[表格：见正文]。在未包裹的静脉内膜中发现大量泡沫细胞，而在包裹的节段中没有或很少见。我们得出结论，通过降低壁应力和扩张，可以预防静脉移植物中的动脉粥样硬化病变。
