Mattila P, Renkonen R
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Biochem Biophys Res Commun. 1992 Dec 30;189(3):1732-8. doi: 10.1016/0006-291x(92)90278-s.
The IFN-gamma linked PKC activation in endothelial cells was analysed. It was shown that IFN-gamma activates PKC in three transient and separate cycles within the first 60 minutes after IFN-gamma stimulation. Before each PKC activation there was an increase in DAG level. IP3, phosphocholine and choline productions were measured to determine the origin of DAG. Neither of the PLC products, IP3 or phosphocholine, were released after IFN-gamma stimulation. On the other hand the PLD products choline and PA were released before all the three activation cycles of PKC.
分析了内皮细胞中与γ干扰素相关的蛋白激酶C（PKC）激活情况。结果显示，在γ干扰素刺激后的最初60分钟内，γ干扰素在三个短暂且独立的周期中激活PKC。每次PKC激活前，二酰甘油（DAG）水平都会升高。通过测量肌醇三磷酸（IP3）、磷酸胆碱和胆碱的生成量来确定DAG的来源。γ干扰素刺激后，磷脂酶C（PLC）的产物IP3或磷酸胆碱均未释放。另一方面，在PKC的所有三个激活周期之前，磷脂酶D（PLD）的产物胆碱和磷脂酸（PA）均已释放。
