Foisy S, Joly E, Sakr F, Levac P, Bibor-Hardy V
Institut du Cancer de Montréal, Que., Canada.
Biochem Cell Biol. 1992 Sep;70(9):758-64. doi: 10.1139/o92-115.
In mammalian tissues, the nuclear lamina is composed of the major lamins A, B, and C, and minor lamins D/E. Although lamin B is present in all cell types, lamins A and C are absent from embryonic cells and most undifferentiated cells from hematopoietic lineage. We have investigated the nuclear lamina protein composition of the Raji cell line, lymphoblast-like cells established from a Burkitt lymphoma patient. Lamins A and C were confirmed absent by immunodetection and Northern blot analysis. Besides lamins B and D/E, a protein migrating around 71 kilodaltons was recognized by a serum directed against the nuclear lamina of BHK-21 fibroblasts. Cellular localization by sequential extraction established this 71-kilodalton protein as an exclusive component of the nuclear lamina fraction. These results indicate that the nuclear lamina has a more complex composition than previously thought to be the case for cells devoid of lamins A and C.
在哺乳动物组织中，核纤层由主要的核纤层蛋白A、B和C以及次要的核纤层蛋白D/E组成。尽管核纤层蛋白B存在于所有细胞类型中，但核纤层蛋白A和C在胚胎细胞以及造血谱系的大多数未分化细胞中不存在。我们研究了Raji细胞系的核纤层蛋白组成，Raji细胞系是从一名伯基特淋巴瘤患者身上建立的类淋巴母细胞。通过免疫检测和Northern印迹分析证实不存在核纤层蛋白A和C。除了核纤层蛋白B和D/E外，一种分子量约为71千道尔顿的蛋白质可被针对BHK-21成纤维细胞核纤层的血清识别。通过顺序提取进行细胞定位确定这种71千道尔顿的蛋白质是核纤层组分的唯一成分。这些结果表明，对于缺乏核纤层蛋白A和C的细胞，核纤层的组成比以前认为的更为复杂。
