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青蛙肌肉肌球蛋白亚片段1和肌动蛋白的制备与表征

Preparation and characterization of frog muscle myosin subfragment 1 and actin.

作者信息

Ferenczi M A, Homsher E, Trentham D R, Weeds A G

出版信息

Biochem J. 1978 Apr 1;171(1):155-63. doi: 10.1042/bj1710155.

DOI:10.1042/bj1710155
PMID:148276
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184145/
Abstract

The preparation, structural and steady-state kinetic characteristics of contractile proteins from the leg muscle of frogs Rana temporaria and Rana pipiens are described. Actin and myosin from the two frog species are indistinguishable. The proteins have structural and steady-state kinetic properties similar to those from rabbit fast-twitch skeletal muscle. Chymotrypsin digestion of frog myosin or myofibrils in the presence of EDTA yields subfragment 1, which is separated by chromatography into two components that are distinguished by their alkali light-chain content.

摘要

描述了来自普通青蛙和豹蛙腿部肌肉收缩蛋白的制备、结构及稳态动力学特征。两种青蛙的肌动蛋白和肌球蛋白无法区分。这些蛋白质具有与兔快肌骨骼肌相似的结构和稳态动力学特性。在乙二胺四乙酸（EDTA）存在的情况下，用胰凝乳蛋白酶消化青蛙肌球蛋白或肌原纤维会产生亚片段1，通过色谱法可将其分离为两个组分，这两个组分可通过其碱性轻链含量来区分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/6e0fdd7e051c/biochemj00489-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/f14bcb8e61ec/biochemj00489-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/4aeba5c144e3/biochemj00489-0163-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/6e0fdd7e051c/biochemj00489-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/f14bcb8e61ec/biochemj00489-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/4aeba5c144e3/biochemj00489-0163-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c1c/1184145/6e0fdd7e051c/biochemj00489-0164-a.jpg

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本文引用的文献

1
Studies on the composition and polymerization of actin.肌动蛋白的组成与聚合研究。
Hung Acta Physiol. 1948;1(4-5):150-63.
2
Determination of serum proteins by means of the biuret reaction.通过双缩脲反应测定血清蛋白。
J Biol Chem. 1949 Feb;177(2):751-66.
3
Chromatography of myosin on diethylaminoethyl-Sephadex A-50.肌球蛋白在二乙氨基乙基-葡聚糖凝胶A-50上的色谱分析。
蛙类骨骼肌肌球蛋白 II 的体外和原位动力学的综合研究。
J Physiol. 2012 Mar 1;590(5):1227-42. doi: 10.1113/jphysiol.2011.222984. Epub 2011 Dec 23.
4
ATPase and shortening rates in frog fast skeletal myofibrils by time-resolved measurements of protein-bound and free Pi.通过对蛋白质结合型和游离型无机磷酸的时间分辨测量，研究青蛙快速骨骼肌肌原纤维中的ATP酶及缩短速率。
Biophys J. 1998 Jun;74(6):3120-30. doi: 10.1016/S0006-3495(98)78018-X.
5
The dependence on extent of shortening of the extra energy liberated by rapidly shortening frog skeletal muscle.青蛙骨骼肌快速缩短所释放的额外能量对缩短程度的依赖性。
J Physiol. 1981 Dec;321:411-22. doi: 10.1113/jphysiol.1981.sp013993.
6
Preparation of frog myosin. Isolation and characterization of the light chains.
J Muscle Res Cell Motil. 1980 Mar;1(1):61-72. doi: 10.1007/BF00711925.
7
Cross-bridge model of muscle contraction. Quantitative analysis.肌肉收缩的横桥模型。定量分析。
Biophys J. 1980 Feb;29(2):195-227. doi: 10.1016/S0006-3495(80)85126-5.
8
Unusual features of the Ca2+-ATPase activity of myosin from fast skeletal muscle of the frog: effect of actin and SH1 thiol group modification.
J Muscle Res Cell Motil. 1983 Apr;4(2):191-206. doi: 10.1007/BF00712030.
9
Comparative study of myosins present in the lateral muscle of some fish: species variations in myosin isoforms and their distribution in red, pink and white muscle.部分鱼类侧肌中肌球蛋白的比较研究：肌球蛋白同工型的物种差异及其在红肌、粉肌和白肌中的分布
J Muscle Res Cell Motil. 1985 Oct;6(5):601-40. doi: 10.1007/BF00711917.
10
Energy stored and dissipated in skeletal muscle basement membranes during sinusoidal oscillations.在正弦振荡期间储存在骨骼肌基底膜中并耗散的能量。
Biophys J. 1986 Dec;50(6):1127-38. doi: 10.1016/S0006-3495(86)83557-3.
Biochemistry. 1967 Feb;6(2):528-40. doi: 10.1021/bi00854a022.
4
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
5
Measurement of protein concentration with interferences optics.利用干涉光学测量蛋白质浓度。
Anal Biochem. 1969 Apr 4;28(1):216-21. doi: 10.1016/0003-2697(69)90172-9.
6
Selective purification of the thiol peptides of myosin.肌球蛋白硫醇肽的选择性纯化。
Biochem J. 1968 Apr;107(4):531-48. doi: 10.1042/bj1070531.
7
Light chains from fast and slow muscle myosins.来自快肌和慢肌肌球蛋白的轻链。
Nature. 1971 Nov 12;234(5324):81-5. doi: 10.1038/234081a0.
8
Control of muscle contraction.肌肉收缩的控制
Q Rev Biophys. 1969 Nov;2(4):351-84. doi: 10.1017/s0033583500001190.
9
The amino-acid sequence of the alkali light chains of rabbit skeletal-muscle myosin.兔骨骼肌肌球蛋白碱性轻链的氨基酸序列。
Eur J Biochem. 1974 May 15;44(2):317-34. doi: 10.1111/j.1432-1033.1974.tb03489.x.
10
Complete amino-acid sequence of actin of rabbit skeletal muscle.兔骨骼肌肌动蛋白的完整氨基酸序列。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2687-91. doi: 10.1073/pnas.70.9.2687.