Orlov S N, Shevchenko A S
Biokhimiia. 1978 Feb;43(2):208-15.
The activities of Ca2+, Mg2+-ATPase and Na+, K+-ATPase and the permeability of reconstituted human erythrocytes for Na and K ions were measured, using Ca2+-EGTA, Ca2+ATP and Ca2+-sodium citrate buffers. It was found that the increase in the Ca2+/chelate ratio caused stimulation of Ca2+, Mg2+- and Na+, K+-Atpases and an increase in the rate constants of ouabain--dependent 42K+ influx and 22Na+ efflux from the erythrocytes. The use of the Ca2+-sodium citrate system as a calcium buffer did not change the parameters of the functional state of erythrocyte membranes. The data obtained are discussed in terms of a possible role of calcium ions, which are bound to the inner surface of the erythrocyte membrane, in the regulation of the systems of active and passive transport of cations.
使用Ca²⁺-EGTA、Ca²⁺ATP和Ca²⁺-柠檬酸钠缓冲液，测定了重组人红细胞中Ca²⁺、Mg²⁺-ATP酶和Na⁺、K⁺-ATP酶的活性以及Na和K离子的通透性。结果发现，Ca²⁺/螯合剂比例的增加会刺激Ca²⁺、Mg²⁺-和Na⁺、K⁺-ATP酶，并增加哇巴因依赖性42K⁺流入和22Na⁺从红细胞流出的速率常数。使用Ca²⁺-柠檬酸钠系统作为钙缓冲液不会改变红细胞膜功能状态的参数。根据与红细胞膜内表面结合的钙离子在阳离子主动和被动转运系统调节中的可能作用，对所得数据进行了讨论。
