Enriquez F J, Boggavarapu J, Bradley-Dunlop D
Hybridoma Technology, University of Arizona, Tucson 85724.
Int Arch Allergy Immunol. 1992;99(1):123-6. doi: 10.1159/000236345.
Unsuccessful attempts to identify serum parasite-specific immunoglobulin E (IgE) responses in mice following infections with the intestinal nematode parasite Heligmosomoides polygyrus prompted us to explore the possibility that IgE bound within the parasite antigen could account for the false-positive results observed. A live-worm ELISA was developed. Following incubation, irrelevant IgE monoclonal antibody to DNP, IgE present in normal mouse serum, as well as IgE in immune serum were independently identified within live adult worms in this H. polygyrus-modified ELISA. It was concluded that in addition to parasite-specific IgE binding to H. polygyrus, the parasite may attract both parasite-specific and non-parasite-specific IgE via non-Fab IgE-binding molecules.
在用肠道线虫寄生虫多房棘球绦虫感染小鼠后，未能成功鉴定出血清寄生虫特异性免疫球蛋白E（IgE）反应，这促使我们探索寄生虫抗原内结合的IgE可能是导致观察到假阳性结果的原因。我们开发了一种活虫ELISA。孵育后，在这种多房棘球绦虫改良的ELISA中，在活的成虫体内分别鉴定出了针对二硝基苯酚（DNP）的无关IgE单克隆抗体、正常小鼠血清中的IgE以及免疫血清中的IgE。得出的结论是，除了寄生虫特异性IgE与多房棘球绦虫结合外，寄生虫可能通过非Fab IgE结合分子吸引寄生虫特异性和非寄生虫特异性IgE。
