Pleass R J, Bianco A E
Department of Pathology, Ninewells Hospital, Dundee, Scotland.
Parasitol Res. 1996;82(5):445-53. doi: 10.1007/s004360050143.
Infections induced in NIH mice by irradiated (300 Gy) larvae of Heligmosomoides polygyrus effectively stimulated immunity to challenge, whereas unirradiated larvae did not. Importantly, this difference was lost by the elimination of the adult worms arising from unirradiated sensitising infections by drug treatment prior to challenge. No difference in the level of parasite-specific serum and mucosal IgG, IgG1, IgG2a, or IgA was detected between immune mice sensitised either with drug-abbreviated unirradiated or irradiated larval infections and non-immune mice receiving two superimposed unirradiated infections. An enzyme-linked immunosorbent assay (ELISA) and immunoblotting data suggested that parasite-specific IgG1 was the predominant antibody class in both serum and intestinal perfusates. IgA exhibited differences in antigen specificity between the serum and the intestine. In serum, IgA responses were directed predominantly to L4 somatic antigens, whereas at the mucosal surface they were biased towards L4 excretory/secretory (ES) antigens. No correlation was found between the intensity of the serum or mucosal antibody responses and the mean worm burdens in groups of immune or non-immune mice. Moreover, no correlation was found between levels of parasite-specific serum or mucosal IgG, IgG1, IgG2a or IgA and the loss of worms in individual mice.
用经300戈瑞照射的多房棘球绦虫幼虫感染NIH小鼠，可有效刺激其对攻击的免疫力，而未经照射的幼虫则不能。重要的是，通过在攻击前用药物治疗消除未经照射的致敏感染产生的成虫，这种差异消失了。在用药物缩短的未经照射或经照射的幼虫感染致敏的免疫小鼠与接受两次叠加的未经照射感染的非免疫小鼠之间，未检测到寄生虫特异性血清和粘膜IgG、IgG1、IgG2a或IgA水平的差异。酶联免疫吸附测定（ELISA）和免疫印迹数据表明，寄生虫特异性IgG1是血清和肠道灌洗液中主要的抗体类别。IgA在血清和肠道之间表现出抗原特异性差异。在血清中，IgA反应主要针对L4体抗原，而在粘膜表面，它们偏向于L4排泄/分泌（ES）抗原。在免疫或非免疫小鼠组中，未发现血清或粘膜抗体反应强度与平均虫负荷之间存在相关性。此外，在个体小鼠中，未发现寄生虫特异性血清或粘膜IgG、IgG1、IgG2a或IgA水平与虫体丢失之间存在相关性。
