关于钾在箭毒及其他药物阻断神经肌肉传递中所起作用的实验。

Experiments on the role of potassium in the blocking of neuromuscular transmission by curare and other drugs.

FENN W O, GERSCHMAN R, FISCHER G, LACY J, BAILLY M D, WRIGHT J L

J Gen Physiol. 1951 May;34(5):607-17. doi: 10.1085/jgp.34.5.607.

Abstract

Experiments with perfused frog muscles and with isolated frog muscles immersed in Ringer's solution have failed to show any effect of curare in liberating potassium from muscle tissue. This makes it difficult to suppose that the paralytic effect of curare can be attributed to cation exchange between curare and K whereby a labile potassium compound needed for stimulation is removed from the neuromuscular junction. 2. Similar negative results were obtained with dihydro-beta-erythroidine and myanesin. 3. A small liberation of K from perfused muscle does result from treatment with acetylcholine. This is probably due to the contracture of the muscle since the effect is largely eliminated by previous treatment of the muscle with curare. The amount of potassium lost in this way from perfused muscles is too small to detect when muscles are analyzed after immersion in Ringer's solution with and without acetylcholine. It is concluded that there is no significant cation exchange between acetylcholine and K in muscle, but only a small loss of K due to the contracture produced by the acetylcholine.

摘要

用灌注蛙肌以及将分离的蛙肌浸于任氏液中的实验均未能显示箭毒对从肌肉组织中释放钾有任何作用。这使得难以设想箭毒的麻痹作用可归因于箭毒与钾之间的阳离子交换，据此，刺激所需的不稳定钾化合物从神经肌肉接头处被移除。2. 用二氢-β-刺桐啶和米那索龙也得到了类似的阴性结果。3. 用乙酰胆碱处理确实会使灌注肌肉有少量钾释放。这可能是由于肌肉收缩，因为用箭毒预先处理肌肉后，该效应大部分被消除。当将肌肉浸于含和不含乙酰胆碱的任氏液中后进行分析时，以这种方式从灌注肌肉中丢失的钾量太少而无法检测到。得出的结论是，在肌肉中乙酰胆碱与钾之间不存在显著的阳离子交换，而只是由于乙酰胆碱产生的收缩导致少量钾丢失。