Zewert T, Harrington M
NSF Center for Molecular Biotechnology, Beckman Institute, Pasadena, CA 91125.
Electrophoresis. 1992 Nov;13(11):817-24. doi: 10.1002/elps.11501301181.
A new series of materials have been tested for their suitability as electrophoresis matrices. The mechanical and optical properties of gels composed of polyethyleneglycol (meth)acrylate esters or polyhydroxy (meth)acrylate esters in water and in various concentrations of organic solvents are described. Several crosslinkers including polyethyleneglycol and polyhydroxy di(meth)acrylates, piperazine diacrylate, and bisacrylamide were used in these studies. Electrophoretic migration and separation of a series of protein standards through polyethyleneglycol methacrylate (PEGM) 200, PEGM 400, and glyceryl methacrylate is demonstrated. Further, copolymerization of all of the monomers with acrylamide was performed and the distribution of monomer incorporation into the polymer network calculated. All monomers and copolymers that were examined by IR spectroscopy showed greater than 99% polymerization. These results justify their further study for biomolecule separations.
已对一系列新材料作为电泳基质的适用性进行了测试。描述了由聚乙二醇（甲基）丙烯酸酯或聚羟基（甲基）丙烯酸酯在水和各种浓度有机溶剂中组成的凝胶的机械和光学性能。这些研究中使用了几种交联剂，包括聚乙二醇和聚羟基二（甲基）丙烯酸酯、哌嗪二丙烯酸酯和双丙烯酰胺。展示了一系列蛋白质标准品通过聚乙二醇甲基丙烯酸酯（PEGM）200、PEGM 400和甲基丙烯酸甘油酯的电泳迁移和分离。此外，还进行了所有单体与丙烯酰胺的共聚反应，并计算了单体在聚合物网络中的掺入分布。通过红外光谱检查的所有单体和共聚物均显示聚合度大于99%。这些结果证明对其进行生物分子分离的进一步研究是合理的。
