Zewert T, Harrington M
NSF Center for Molecular Biotechnology, Beckman Institute, Pasadena, CA 91125.
Electrophoresis. 1992 Nov;13(11):824-31. doi: 10.1002/elps.11501301182.
The properties of gels composed of poly(polyethyleneglycol methacrylate) and copolymers of polyethyleneglycol methacrylate and acrylamide were studied. These novel electrophoresis matrices are amphigels (swellable in water and organic solvents) that have unprecedented organic solvent compatibility. Hydrophobic proteins which are poorly solubilized in aqueous detergent systems (e.g., zein) are well-resolved in these gels with hydro-organic solvents. This is especially relevant for isoelectric focusing, to avoid using either ionic detergents that may interfere with the focusing, or urea that may cause carbamylation of proteins. Variations of crosslinker, buffer, and solvent systems in these gels were explored.
研究了由聚（聚乙二醇甲基丙烯酸酯）以及聚乙二醇甲基丙烯酸酯与丙烯酰胺的共聚物组成的凝胶的性质。这些新型电泳基质是两性凝胶（可在水和有机溶剂中溶胀），具有前所未有的有机溶剂兼容性。在水性去污剂体系中溶解性较差的疏水蛋白质（例如玉米醇溶蛋白）在含有水-有机溶剂的这些凝胶中能得到很好的分离。这对于等电聚焦尤为重要，可避免使用可能干扰聚焦的离子去污剂或可能导致蛋白质氨甲酰化的尿素。还探索了这些凝胶中交联剂、缓冲液和溶剂体系的变化。
