van den Berg B M, Tamboer J H
Royal Sluis Holding B. V., Biotech Center, Section Plant Biochemistry, Enkhuizen, The Netherlands.
Electrophoresis. 1992 Nov;13(11):880-1. doi: 10.1002/elps.11501301192.
An apparatus was developed to homogenize 96 individual seeds of tomato, pepper, cabbages, and other seeds of similar size in a 96-well tissue culture plate in 45 s. The apparatus, named the terminator, consists of two parts: a base plate that can hold the 96-well tissue culture plate, and a vibrating part that is attached to a variable transformer. The seeds are homogenized by the vibrating action of 96 small homogenizers that are attached to the vibrating part and that fit perfectly into the wells of the tissue culture plate. The seed homogenates made in this way can be used for electrophoretic analysis of genetic variations of seed proteins.
开发了一种装置,可在45秒内将96颗番茄、辣椒、卷心菜种子以及其他大小相似的种子在96孔组织培养板中进行均质化处理。该装置名为终结者,由两部分组成:一个可容纳96孔组织培养板的底板,以及一个连接到可变变压器的振动部件。通过连接在振动部件上并完美适配于组织培养板孔的96个小型均质器的振动作用来使种子均质化。以这种方式制备的种子匀浆可用于种子蛋白遗传变异的电泳分析。