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C-value paradox in angiosperm plant species. I. Sensitivity to DNase I in species with different 2C DNA content.

作者信息

Olszewska M J

机构信息

Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.

出版信息

Folia Histochem Cytobiol. 1992;30(2):41-8.

PMID:1483532
Abstract

The experimental conditions for DNAase I digestion in situ for plant nuclei have been presented. Cytophotometric measurements of DNA loss performed on Feulgen-stained nuclei of three species differing in 2C DNA, heterochromatin and condensed euchromatin contents have shown that the lower 2C DNA amount the higher is DNase I sensitivity. Heterochromatin and some fractions of euchromatin are DNase I resistant. Microdensitometric measurements along M chromosome in Vicia faba have demonstrated the sites hypersensitive to DNase I.

摘要

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