Gabara B, Wojtyła-Kuchta B, Tarczyńska M
Department of Cytology and Cytochemistry, University of Lódź, Poland.
Folia Histochem Cytobiol. 1992;30(2):69-73.
Cortex cells of the meristematic (1 mm) and differentiated (7 mm) zones of Pisus sativus L. roots after 144 h culture in distilled water (control), Ca2+ (10(-3) M) and/or Cd2+, Cr3+, Pb2+ solutions (10(-4) M, each) were subject of the present studies. Reductions in the number of nuclei incorporating 3H thymidine was observed in meristem in the presence of Cd2+ and in differentiated zone in Pb2+ treated roots. Intensity of DNA synthesis diminished after Pb2+ treatment but mostly in Cd2+ treated roots, mainly in meristem. Addition of calcium to the metal solutions caused an increase in the number of nuclei witch uptook the radioactive material in both studied root zones. Positive effect of calcium alone was visible also in the differentiated zone. Presence of calcium in the metal solutions caused a marked increase in 3H thymidine incorporation into nuclear DNA in meristem, although neutralizing calcium effect in this root zone was visible only in roots treated with Cr2+ and Pb2+. In the differentiated zone of roots growing in Cd2+ solution, calcium addition stimulated DNA synthesis but the intensity of this process was lower than in control (water).
将豌豆（Pisus sativus L.）根的分生组织区（1毫米）和分化区（7毫米）的皮层细胞在蒸馏水（对照）、Ca2+（10(-3)M）和/或Cd2+、Cr3+、Pb2+溶液（各10(-4)M）中培养144小时后作为本研究的对象。在Cd2+存在下，分生组织中掺入3H胸腺嘧啶核苷的细胞核数量减少，在Pb2+处理的根的分化区也出现这种情况。Pb2+处理后DNA合成强度降低，但在Cd2+处理的根中降低更为明显，主要是在分生组织中。向金属溶液中添加钙导致两个研究根区中摄取放射性物质的细胞核数量增加。单独的钙在分化区也有明显的积极作用。金属溶液中钙的存在导致分生组织中3H胸腺嘧啶核苷掺入核DNA的量显著增加，尽管只有在用Cr2+和Pb2+处理的根中，钙在该根区的中和作用才可见。在Cd2+溶液中生长的根的分化区，添加钙刺激了DNA合成，但该过程的强度低于对照（水）。
