Payá M, Halliwell B, Hoult J R
Division of Biomedical Sciences, King's College London, UK.
Free Radic Res Commun. 1992;17(5):293-8. doi: 10.3109/10715769209079522.
Sixteen plant-derived or synthetic coumarins with various hydroxyl and other substitutions were tested for their ability to scavenge alkylperoxyl radicals generated in the aqueous phase by the controlled thermolysis of 2,2'-azo-bis-(2-amidinopropane) dihydrochloride (ABAP). Protection by coumarins against inactivation of lysozyme by the radicals was assayed by measuring the loss of turbidity of suspensions of M. lysodeikticus. Ten of the coumarins were potent scavengers of aqueous peroxyl radicals with activities comparable to n-propyl gallate, desferrioxamine, ferrioxamine and trolox c, yielding IC50 values in the range 21 to 92 micromolar. The presence of 6,7-ortho-dihydroxy functions gave compounds of the greatest potency. Scavenging activity was unrelated to ability to chelate iron ions. The active coumarins are attractive candidates for evaluation as protective agents against disorders in which oxidative stress is implicated.
对十六种具有不同羟基及其他取代基的植物源或合成香豆素进行了测试，以考察它们清除由2,2'-偶氮双-(2-脒基丙烷)二盐酸盐(ABAP)的受控热解在水相中产生的烷基过氧自由基的能力。通过测量溶壁微球菌悬浮液的浊度损失，测定了香豆素对自由基导致的溶菌酶失活的保护作用。其中十种香豆素是水相过氧自由基的有效清除剂，其活性与没食子酸正丙酯、去铁胺、铁胺和生育三烯酚c相当，IC50值在21至92微摩尔范围内。6,7-邻二羟基官能团的存在使化合物具有最大的效力。清除活性与螯合铁离子的能力无关。这些具有活性的香豆素作为针对涉及氧化应激的疾病的保护剂进行评估具有吸引力。
