Asakura T, Sano N, Shichi H
Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan.
J Ocul Pharmacol. 1992 Winter;8(4):333-41. doi: 10.1089/jop.1992.8.333.
The activity to synthesize prostaglandins (PGs*) from arachidonic acid [i.e. cyclooxygenase (CO) activity] was compared between nonpigmented epithelial (NPE) cells and pigmented epithelial (PE) cells from porcine ciliary processes. PG synthesizing activities were about the same (0.58 and 0.52 pmol per mg protein per 15 min for NPE and PE, respectively), and inhibited by indomethacin and anti-CO antibody but not by nordihydroguaiaretic acid. By immunogold labeling, CO immunoreactivity was found both in NPE and PE. The present results and the previous observation on higher PG omega/omega-1 hydroxylation activities in NPE than in PE, taken together, suggest that controlled synthesis and inactivation of PGs may have a role in functions of NPE. When PGE2 was incubated with NPE or PE, NPE cells accumulated PGE2 more actively than did PE cells. PG uptake by the NPE cells may prevent aqueous and vitreous PGs from entering the anterior chamber and causing anterior uveitis.
比较了猪睫状体非色素上皮（NPE）细胞和色素上皮（PE）细胞中从花生四烯酸合成前列腺素（PGs*）的活性[即环氧化酶（CO）活性]。PG合成活性大致相同（NPE和PE分别为每毫克蛋白质每15分钟0.58和0.52皮摩尔），并被吲哚美辛和抗CO抗体抑制，但不被去甲二氢愈创木酸抑制。通过免疫金标记，在NPE和PE中均发现了CO免疫反应性。本研究结果与之前观察到的NPE中PG ω/ω-1羟化活性高于PE的结果相结合，表明PGs的受控合成和失活可能在NPE的功能中起作用。当将前列腺素E2（PGE2）与NPE或PE一起孵育时，NPE细胞比PE细胞更积极地积累PGE2。NPE细胞对PG的摄取可能会阻止房水和玻璃体中的PG进入前房并引起前葡萄膜炎。
