Maurel D, Mas N, Roch G, Boissin J, Arendt J
URA-CNRS 1197, Université de Montpellier II, France.
J Pineal Res. 1992 Oct;13(3):117-23. doi: 10.1111/j.1600-079x.1992.tb00065.x.
The existence of the major urinary metabolite of melatonin, 6-sulphatoxymelatonin (aMT6s), was validated for mink and the 24 hr urinary excretion pattern was determined in intact and superior cervical ganglionectomized animals under different photoperiodic conditions. Within- and between-assay variations, parallelism between serially mid-night pooled urine dilutions and standard curves in aMT6s free urine of mink at 1:125 dilution and recovery of aMT6s in mid-day pooled urine at 1:125 dilution provided a good validation for the mink urinary a MT6s assay. In natural photoperiods (January, LD 9:15; April, LD 13:11) the diurnal rhythm was characterized by low aMT6s values during the day and high values at night. There were no differences in the nocturnal values measured under long- (April, 4.11 +/- 0.40 ng/hr) or short-day (January, 4.74 +/- 0.36 ng/hr) conditions. In an experimental long photoperiod (LD 15:9), the same result was obtained on the 24 hr rhythm in intact animals, but in ganglionectomized mink the nocturnal rise in aMT6s was abolished and the nocturnal values were always low (0.88 +/- 0.09 ng/hr). Our results agree with those obtained in other species concerning plasma melatonin rhythm and urinary aMT6s excretion; we thus conclude that this is an effective assay for measuring pineal activity in mink.
褪黑素的主要尿液代谢产物6-硫酸氧褪黑素（aMT6s）在水貂体内的存在得到了验证，并在不同光周期条件下，测定了完整和颈上神经节切除动物的24小时尿液排泄模式。水貂尿液中aMT6s检测的批内和批间变异、连续午夜混合尿液稀释液与1:125稀释度的水貂无aMT6s尿液标准曲线之间的平行性以及1:125稀释度的中午混合尿液中aMT6s的回收率，为水貂尿液aMT6s检测提供了良好的验证。在自然光照周期（1月，光暗比9:15；4月，光暗比13:11）下，昼夜节律的特点是白天aMT6s值低，夜间值高。在长日照（4月，4.11±0.40 ng/小时）或短日照（1月，4.74±0.36 ng/小时）条件下测得的夜间值没有差异。在实验性长光照周期（光暗比15:9）下，完整动物的24小时节律得到了相同的结果，但在神经节切除的水貂中，aMT6s的夜间升高被消除，夜间值始终较低（0.88±0.09 ng/小时）。我们的结果与在其他物种中获得的关于血浆褪黑素节律和尿液aMT6s排泄结果一致；因此我们得出结论，这是一种测量水貂松果体活性的有效检测方法。
