[从具有新型抗原的福氏志贺菌88 - 893中分离的脂多糖的化学和血清学研究]

[Chemical and serological study of lipopolysaccharide isolated from Shigella flexneri 88-893 possessing a new type-antigen].

作者信息

Toshima Y, Iguchi T, Kondo S, Matsushita S, Kudoh Y, Hisatsune K

机构信息

Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.

出版信息

Nihon Saikingaku Zasshi. 1992 Nov;47(6):777-81. doi: 10.3412/jsb.47.777.

DOI:10.3412/jsb.47.777

PMID:1484404

Abstract

The O-specific polysaccharide chain which represents a new type-antigen in lipopolysaccharide (LPS) of Shigella flexneri 88-893 was investigated. The O-polysaccharide chain was found to be composed of repeating units comprising rhamnose, N-acetylglucosamine and glucose (3:1:2). In the passive hemolysis test, group-6 antiserum of S. flexneri exhibited a high hemolytic titer (50% hemolysis titer: 7,900) against sheep red blood cells (SRBC) sensitized with intact 893 LPS, but virtually no hemolytic activity against SRBC sensitized with alkali-treated 893 LPS. None of the type-specific antisera (I-VI), showed any significant hemolytic titer against SRBC sensitized with either intact or alkali-treated 893 LPS. Thus, 893 LPS contained both the group-6 antigen and a new type-antigen which is distinct from any known type-antigen of S. flexneri.

摘要

对代表福氏志贺菌88 - 893脂多糖（LPS）中一种新型抗原的O-特异性多糖链进行了研究。发现O-多糖链由包含鼠李糖、N-乙酰葡糖胺和葡萄糖（3:1:2）的重复单元组成。在被动溶血试验中，福氏志贺菌6组抗血清对用完整893 LPS致敏的绵羊红细胞（SRBC）表现出高溶血效价（50%溶血效价：7900），但对用碱处理的893 LPS致敏的SRBC几乎没有溶血活性。任何一种型特异性抗血清（I - VI）对用完整或碱处理的893 LPS致敏的SRBC均未表现出任何显著的溶血效价。因此，893 LPS既含有6组抗原，也含有一种与福氏志贺菌任何已知型抗原不同的新型抗原。