组织培养基；人胎盘脐带血清中的必需非透析因子。
Tissue culture media; the essential non-dialyzable factors in human placental cord serum.
作者信息
JACQUEZ J A, BARRY E
出版信息
J Gen Physiol. 1951 Jul;34(6):765-74. doi: 10.1085/jgp.34.6.765.
Abstract
- Human placental cord serum contains a factor or factors necessary for active growth of fibroblasts in tissue culture and a factor which prevents the appearance of a characteristic toxicity. 2. The growth-promoting factor(s) is stable to heating to 60 degrees C. for 30 minutes and is associated with the euglobulin fraction of the serum. 3. Extraction of dried cord serum with benzene, chloroform, and ether does not cause any decrease in growth-promoting activity. 4. Sera from hypophysectomized, adrenalectomized, and thyroid-parathyroidectomized rats show as much growth-promoting activity as normal rat sera. 5. Bovine gamma globulins, anterior pituitary growth hormone, and insulin do not replace the serum growth factor. 6. The toxicity-preventing factor is serum albumin. 7. Evidence is presented that the toxicity is due to free fatty acids in the medium and that serum albumin acts by binding the fatty acids.
摘要
- 人胎盘脐带血清含有组织培养中成纤维细胞活跃生长所需的一种或多种因子，以及一种可防止出现特征性毒性的因子。2. 生长促进因子在60℃加热30分钟后仍稳定，且与血清的优球蛋白部分相关。3. 用苯、氯仿和乙醚提取干燥的脐带血清不会导致生长促进活性降低。4. 垂体切除、肾上腺切除和甲状腺-甲状旁腺切除大鼠的血清显示出与正常大鼠血清一样多的生长促进活性。5. 牛γ球蛋白、垂体前叶生长激素和胰岛素不能替代血清生长因子。6. 毒性预防因子是血清白蛋白。7. 有证据表明毒性是由于培养基中的游离脂肪酸所致，血清白蛋白通过结合脂肪酸起作用。
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