Kondo E, Yoshino T, Akagi T, Hayashi K, Takahashi K
Second Department of Pathology, Okayama University Medical School, Japan.
Acta Med Okayama. 1992 Dec;46(6):407-15. doi: 10.18926/AMO/32636.
Southern blot hybridization was used to detect the rearrangement and amplification of five proto-oncogenes (bcl-2, bcl-1, c-myc, c-myb and c-Ha-ras) and one tumor suppressor gene (RB-1) in 55 Japanese patients with non-Hodgkin's lymphoma; 16 with T-cell lymphomas and 39 with B-cell lymphomas (7 follicular and 32 diffuse lymphomas). Genetic abnormalities of the proto-oncogenes were detected in 7 of the 55 (13%). Genetic abnormalities of bcl-2 plus other genes were detected in 5 of 7 cases of follicular lymphoma (71%), rearrangements of bcl-2 and c-myc, rearrangement of bcl-2 and amplification of c-myb. Genetic abnormalities were observed in only three cases of diffuse lymphoma. In each of 3 cases of B-cell lymphoma, one of the genes, blc-2 mbr, bcl-2 mcr and c-myc, was rearranged respectively. The incidence of genetic abnormalities in diffuse lymphomas (6.3%) was lower than that in follicular lymphomas. None of diffuse lymphomas had double oncogene abnormality. No abnormalities were found in RB-1, bcl-1, and Ha-ras. These findings suggest that follicular lymphomas are associated with some abnormalities of oncogenes not restricted to bcl-2 that facilitate growth which may be associated with their clinical features.
采用Southern印迹杂交法检测55例日本非霍奇金淋巴瘤患者中5种原癌基因（bcl - 2、bcl - 1、c - myc、c - myb和c - Ha - ras）和1种肿瘤抑制基因（RB - 1）的重排与扩增情况；其中16例为T细胞淋巴瘤患者，39例为B细胞淋巴瘤患者（7例滤泡性淋巴瘤和32例弥漫性淋巴瘤）。55例患者中有7例（13%）检测到原癌基因的遗传异常。7例滤泡性淋巴瘤中有5例（71%）检测到bcl - 2及其他基因的遗传异常，包括bcl - 2和c - myc重排、bcl - 2重排及c - myb扩增。仅在3例弥漫性淋巴瘤中观察到遗传异常。在3例B细胞淋巴瘤中，分别有1例blc - 2 mbr、bcl - 2 mcr和c - myc基因发生重排。弥漫性淋巴瘤的遗传异常发生率（6.3%）低于滤泡性淋巴瘤。弥漫性淋巴瘤均无双重原癌基因异常。在RB - 1、bcl - 1和Ha - ras基因中未发现异常。这些发现提示，滤泡性淋巴瘤与某些癌基因异常有关，不限于bcl - 2，这些异常促进肿瘤生长，可能与其临床特征相关。
