Ridet J L, Sandillon F, Rajaofetra N, Geffard M, Privat A
INSERM U. 336-EPHE, Université Montpellier II, France.
Brain Res. 1992 Dec 11;598(1-2):233-41. doi: 10.1016/0006-8993(92)90188-f.
The mapping of the spinal dopaminergic innervation has been performed in the adult rat using an anti-dopamine antiserum. Immunoreactive fibers were detected with the light microscope in the dorsal horn (mainly in laminae III-IV), in the intermediolateral cell column (IML), in the peri-ependymal region and in the ventral horn. The ultrastructural analysis of dopaminergic innervation showed mainly axodendritic contacts and fewer axosomatic ones. In the ventral horn and the IML, the pattern of dopaminergic innervation exhibited a majority of classical synapses. In the dorsal horn, dopaminergic innervation was partly non-synaptic (at cervical level), whereas numerous axodendritic synapses were observed at thoraco-lumbar level. Previous studies described the non-synaptic organization of serotonergic and noradrenergic projections in the dorsal horn. It is thus hypothesized that the monoaminergic systems, involved in pain modulation within the dorsal horn, act partly through volume transmission. In contrast, these systems would modulate the motor and autonomic functions through classical synapses.
利用抗多巴胺抗血清对成年大鼠的脊髓多巴胺能神经支配进行了定位。在光镜下，在背角（主要在Ⅲ-Ⅳ层）、中间外侧细胞柱（IML）、室管膜周围区域和腹角检测到免疫反应性纤维。多巴胺能神经支配的超微结构分析显示，主要是轴突-树突接触，轴突-胞体接触较少。在腹角和IML，多巴胺能神经支配模式表现为大多数经典突触。在背角，多巴胺能神经支配部分为非突触性（在颈部水平），而在胸腰段水平观察到大量轴突-树突突触。先前的研究描述了背角中5-羟色胺能和去甲肾上腺素能投射的非突触组织。因此推测，参与背角内疼痛调节的单胺能系统部分通过容积传递起作用。相比之下，这些系统将通过经典突触调节运动和自主功能。
