Tanii I, Toshimori K, Araki S, Oura C
Department of Anatomy, Miyazaki Medical College, Japan.
Cell Tissue Res. 1992 Dec;270(3):451-7. doi: 10.1007/BF00645046.
A monoclonal antibody (MC41) was produced that specifically recognizes a sperm acrosomal antigen of approximately 165,000 dalton in the rat. Rat testis was examined using a pre-embedding immunoperoxidase technique to reveal the pathway of the MC41 antigen to the acrosome during spermiogenesis. The MC41 immunoreaction appeared in several organelles of spermatids in a stage-specific manner: (1) in the endoplasmic reticulum (ER) throughout spermiogenesis, (2) in the outer acrosomal membrane from steps 9 to 19, (3) as a weak immunoreaction in the vesicular structures in the acrosomal matrix from steps 11 to 17, and (4) as a strong immunoreaction in the acrosomal matrix especially at the terminal step of spermiogenesis (step 19). However, no immunoreaction was observed in the Golgi region throughout spermiogenesis. These results suggest that the pathway of the MC41 antigen leads firstly from the ER to the outer acrosomal membrane and secondly to the acrosomal matrix. This pathway does not involve the Golgi apparatus and is referred to as the "extra-Golgi pathway".
制备了一种单克隆抗体（MC41），它能特异性识别大鼠中一种分子量约为165,000道尔顿的精子顶体抗原。利用包埋前免疫过氧化物酶技术检查大鼠睾丸，以揭示精子发生过程中MC41抗原向顶体的转运途径。MC41免疫反应以阶段特异性方式出现在精子细胞的几种细胞器中：（1）在整个精子发生过程中出现在内质网（ER）中；（2）在第9至19步出现在顶体外膜中；（3）在第11至17步作为顶体基质中囊泡结构的弱阳性反应；（4）在顶体基质中尤其是在精子发生的最后一步（第19步）作为强阳性反应。然而，在整个精子发生过程中，高尔基体区域未观察到免疫反应。这些结果表明，MC41抗原的转运途径首先是从内质网到顶体外膜，其次是到顶体基质。该途径不涉及高尔基体，被称为“高尔基体外途径”。
