含人5号染色体白细胞介素基因的酵母人工染色体的特性分析。

Characterization of yeast artificial chromosomes containing interleukin genes on human chromosome 5.

作者信息

Stock W, Chandrasekharappa S C, Neuman W L, Le Beau M M, Brownstein B H, Westbrook C A

机构信息

Department of Medicine, University of Chicago, IL 60637.

出版信息

Cytogenet Cell Genet. 1992;61(4):263-5. doi: 10.1159/000133417.

DOI:10.1159/000133417

PMID:1486800

Abstract

To understand better the organization and linkage of the interleukin genes, IL4 and IL5, we prepared long-range restriction maps of five yeast artificial chromosomes (YACs) containing IL5. We determined that IL4 and IL5 are within 100-170 kb, and that the regions surrounding these genes contain several GC-rich areas. Fluorescence in situ chromosomal analysis demonstrated that three of the five YAC clones contain non-contiguous genomic sequences originating from multiple human chromosomes.

摘要

为了更好地理解白细胞介素基因IL4和IL5的组织和连锁关系，我们制备了包含IL5的五个酵母人工染色体（YAC）的长程限制性图谱。我们确定IL4和IL5位于100 - 170 kb范围内，并且这些基因周围的区域包含几个富含GC的区域。荧光原位染色体分析表明，五个YAC克隆中的三个包含源自多个人类染色体的不连续基因组序列。