一种用于杜克嗜血杆菌初代分离的简易培养基。

A simple medium for the primary isolation of Haemophilus ducreyi.

作者信息

Dangor Y, Miller S D, Koornhof H J, Ballard R C

机构信息

Emergent Pathogen Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg, Republic of South Africa.

出版信息

Eur J Clin Microbiol Infect Dis. 1992 Oct;11(10):930-4. doi: 10.1007/BF01962377.

DOI:10.1007/BF01962377

PMID:1486890

Abstract

Two simple, inexpensive media containing gonococcal agar-base, supplemented with 5% Fildes' extract and either chocolated or unchocolated horse blood (GC-FHBC or GC-FHB) were compared with the standard gonococcal agar-based (GC-HgS) and Mueller-Hinton agar-based media (MH-HB) normally used for primary isolation of Haemophilus ducreyi from presumptive chancroid lesions. Overall, Haemophilus ducreyi was recovered from 162 of 178 (91%) samples from primary chancroid lesions. As a single isolation medium GC-HgS proved the most sensitive with an isolation rate of 80% followed by GC-FHB (75%), MH-HB and GC-FHC (both 71%). Use of a combination of GC-HgS and MH-HB resulted in isolation of Haemophilus ducreyi in 160 of 178 cases (90%). Since GC-FHB is approximately one-quarter the cost of the combination and half the cost of GC-HgS or MH-HB alone, this medium could prove suitable for diagnostic purposes in developing countries where chancroid is endemic.

摘要

将两种简单、廉价的含淋球菌琼脂基础培养基进行比较，这两种培养基添加了5%的费氏提取物以及巧克力化或未巧克力化的马血（GC-FHBC或GC-FHB），与通常用于从疑似软下疳病变中初次分离杜克雷嗜血杆菌的标准含淋球菌琼脂基础培养基（GC-HgS）和含穆勒-欣顿琼脂基础培养基（MH-HB）进行比较。总体而言，从178份原发性软下疳病变样本中的162份（91%）中分离出了杜克雷嗜血杆菌。作为单一分离培养基，GC-HgS最为敏感，分离率为80%，其次是GC-FHB（75%）、MH-HB和GC-FHC（均为71%）。使用GC-HgS和MH-HB的组合在178例病例中的160例（90%）中分离出了杜克雷嗜血杆菌。由于GC-FHB的成本约为该组合的四分之一，是单独使用GC-HgS或MH-HB成本的一半，这种培养基在软下疳流行的发展中国家可能适合用于诊断目的。