Dylewski J, Nsanze H, Maitha G, Ronald A
Diagn Microbiol Infect Dis. 1986 Mar;4(3):241-5. doi: 10.1016/0732-8893(86)90103-3.
Laboratory confirmation of the clinical diagnosis of chancroid requires the isolation of Haemophilus ducreyi. Enriched gonococcal (GcHbS) and Mueller-Hinton agar (MHHb) both support the growth of most strains of H. ducreyi. In this study we compared the isolation rate of H. ducreyi on GcHbs and MHHb media in 111 men with genital ulcer disease. A second culture was obtained in 84 men at 48 hr in order to determine the reproducibility of H. ducreyi culture. The sensitivity of a single and a sequential pair of cultures on GcHbS was 67% and 74%, respectively, on men with presumed chancroid. The sensitivity of the MHHb as a single culture was 53%. Using both media for the initial culture of genital ulcers, and repeating the culture on GcHbs at 48 hr, increased the sensitivity of the isolation of H. ducreyi to 92% in men who had no prior antimicrobial use and who had no laboratory evidence of primary syphilis.
软下疳临床诊断的实验室确诊需要分离出杜克雷嗜血杆菌。富集淋病奈瑟菌（GcHbS）琼脂和穆勒-欣顿琼脂（MHHb）均支持大多数杜克雷嗜血杆菌菌株的生长。在本研究中，我们比较了111例患有生殖器溃疡疾病的男性中，杜克雷嗜血杆菌在GcHbs和MHHb培养基上的分离率。为了确定杜克雷嗜血杆菌培养的可重复性，对84名男性在48小时时进行了第二次培养。对于疑似软下疳的男性，在GcHbS上进行单次培养和连续两次培养的敏感性分别为67%和74%。MHHb作为单次培养的敏感性为53%。对生殖器溃疡进行初次培养时同时使用两种培养基，并在48小时时在GcHbs上重复培养，可使未使用过抗菌药物且无一期梅毒实验室证据的男性中杜克雷嗜血杆菌的分离敏感性提高到92%。
