多氯联苯Aroclor 1254对大鼠附睾的影响。

Effect of polychlorinated biphenyl, Aroclor 1254 on rat epididymis.

作者信息

Anbalagan J, Kanagaraj P, Srinivasan N, Aruldhas M M, Arunakaran J

机构信息

Department of Endocrinology, Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.

出版信息

Indian J Med Res. 2003 Dec;118:236-42.

PMID:14870796

Abstract

BACKGROUND & OBJECTIVES: Polychlorinated biphenyls (PCB), the synthetic chlorinated organic compounds, are known to decrease thyroid function, sperm count and fertility, and increase the risk of testicular cancer may cause serious effect on male reproduction. The objective of the present study was to study the effect of PCB, Aroclor 1254 on rat epididymal structure and function.

METHODS

Adult male albino rats were treated ip with Aroclor 1254, 200 microg/kg body weight for 15 and 30 days. Serum levels of testosterone, estradiol, total triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) were measured by radioimmunoassay. The epididymal weight, sperm count, and caudal epididymal sialic acid, glyceryl phosphoryl choline (GPC) were also investigated. Histological studies were done on caput and caudal epididymal regions.

RESULTS

Serum testosterone showed no change, but estradiol levels increased in 30 days treated animals, T3 and T4 levels decreased and TSH levels increased in both 15 and 30 days treated animals. Body weight, epididymal weight, sialic acid, GPC and sperm count were decreased only in 30 days Aroclor treated group.

INTERPRETATION & CONCLUSION: The results suggested that Aroclor 1254 treatment for 15 and 30 days induced hypothyroidism in rats, but epididymal functions were altered only at 30 days treatment. The adverse effect of Aroclor 1254 (PCB) on epididymis might be due to indirect action through hormonal regulation.

摘要

背景与目的

多氯联苯（PCB）是合成的含氯有机化合物，已知其会降低甲状腺功能、精子数量和生育能力，并增加患睾丸癌的风险，可能对男性生殖造成严重影响。本研究的目的是研究PCB（商品名Aroclor 1254）对大鼠附睾结构和功能的影响。

方法

成年雄性白化大鼠腹腔注射Aroclor 1254，剂量为200微克/千克体重，持续15天和30天。采用放射免疫分析法测定血清睾酮、雌二醇、总三碘甲状腺原氨酸（T3）、甲状腺素（T4）和促甲状腺激素（TSH）水平。还研究了附睾重量、精子数量以及附睾尾部的唾液酸、甘油磷酸胆碱（GPC）。对附睾头部和尾部区域进行了组织学研究。