Hasséus B, Jontell M, Bergenholtz G, Dahlgren U I
Department of Endodontology/Oral Diagnosis/Oral Medicine, Faculty of Odontology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Eur J Oral Sci. 2004 Feb;112(1):48-54. doi: 10.1111/j.0909-8836.2004.00097.x.
Oral Langerhans cells (LC) have better T-cell costimulatory capacity than skin LC. In this study factors affecting this capacity have been assessed in a mixed epithelial cell lymphocyte reaction (MELR) assay. Flow cytometry analysis of freshly recovered cells revealed major histocompatibility complex (MHC) class II molecule expression on 7.5% of the oral epithelial cells and 9.7% of the skin epithelial cells. Monoclonal anti class II antibodies significantly reduced the T-cell proliferation in the MELR. Pretreatment of skin epithelial cells with interleukin-1beta, tumour necrosis factor-alpha or interferon (IFN)-gamma did not affect the MELR proliferation, but incubation with IFNgamma significantly suppressed the T-cell response. Transfer of supernatants from cultures of skin epithelial cells and allogeneic T cells to cultures of oral epithelial cells and T cells resulted in a reduced T-cell proliferation while supernatants from oral epithelial cells and T cells did not reduce proliferation. The higher proliferation in cultures of T cells and oral epithelial cells than in cultures containing skin epithelial cells may be due to the presence of a suppressive factor in the skin epithelial cell suspensions.
口腔朗格汉斯细胞（LC）比皮肤LC具有更好的T细胞共刺激能力。在本研究中，通过混合上皮细胞淋巴细胞反应（MELR）试验评估了影响这种能力的因素。对新鲜回收细胞的流式细胞术分析显示，7.5%的口腔上皮细胞和9.7%的皮肤上皮细胞表达主要组织相容性复合体（MHC）II类分子。单克隆抗II类抗体显著降低了MELR中的T细胞增殖。用白细胞介素-1β、肿瘤坏死因子-α或干扰素（IFN）-γ预处理皮肤上皮细胞不影响MELR增殖，但与IFNγ孵育显著抑制T细胞反应。将皮肤上皮细胞和同种异体T细胞培养物的上清液转移至口腔上皮细胞和T细胞培养物中导致T细胞增殖减少，而口腔上皮细胞和T细胞培养物的上清液则不降低增殖。T细胞与口腔上皮细胞培养物中的增殖高于含有皮肤上皮细胞的培养物，这可能是由于皮肤上皮细胞悬液中存在抑制因子。
