Siedlar M, Uracz W, Zembala M
Department of Clinical Immunology, Copernicus Medical School, Cracow, Poland.
Immunol Lett. 1992 Dec;34(3):249-56. doi: 10.1016/0165-2478(92)90221-9.
This paper describes a simple kinetic colorimetric assay for the quantitation of human peripheral blood monocyte-mediated cytotoxic activity against tumour cells. Isolated effector monocytes were cultured overnight with an increasing number of target cells in 96-well microplates. Cytotoxic activity of monocytes was determined by modified nitroblue tetrazolium (MTT) dye assay using standard ELISA reader offering possible automation. The test was performed with three different effector/target cell ratios using a fixed number of monocytes. This allowed the expression of cytotoxic activity of monocytes in cytotoxic activity units. The assay was found to be a simple method to demonstrate that low doses of TNF (1 U/ml) enhanced monocyte-mediated cytotoxicity.
本文描述了一种简单的动力学比色测定法，用于定量检测人外周血单核细胞介导的针对肿瘤细胞的细胞毒性活性。将分离出的效应单核细胞与数量不断增加的靶细胞在96孔微孔板中培养过夜。使用可实现自动化的标准酶标仪，通过改良的硝基蓝四氮唑（MTT）染料测定法来确定单核细胞的细胞毒性活性。使用固定数量的单核细胞，以三种不同的效应细胞/靶细胞比例进行该试验。这使得能够以细胞毒性活性单位来表达单核细胞的细胞毒性活性。该测定法被发现是一种简单的方法，可证明低剂量的肿瘤坏死因子（1 U/ml）可增强单核细胞介导的细胞毒性。
