Liu J S, Molchanova T P, Gu L H, Wilson J B, Hopmeier P, Schnedl W, Balaun E, Krejs G J, Huisman T H
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
Hemoglobin. 1992;16(6):493-501. doi: 10.3109/03630269208993117.
Two abnormal hemoglobins were accidentally detected by cation exchange high performance liquid chromatography with the Diamat system of Bio-Rad Laboratories; the variants eluted together with the fast-moving Hb A1c. Structural analysis of isolated beta chains and sequence analysis of amplified DNA identified a new variant, i.e. Hb Graz that has a His-->Leu replacement at position 2 of the beta chain, in four healthy, apparently unrelated, adults. The second variant was identical to Hb Sherwood Forest or alpha 2 beta 2(104)(G6)Arg-->Thr; it is believed that this may be the second observation of this abnormal hemoglobin.
使用伯乐公司的Diamat系统通过阳离子交换高效液相色谱法意外检测到两种异常血红蛋白；这些变体与快速移动的糖化血红蛋白A1c一起洗脱。对分离出的β链进行结构分析以及对扩增的DNA进行序列分析，在四名健康、看似无亲缘关系的成年人中鉴定出一种新变体，即β链第2位发生组氨酸→亮氨酸替代的格拉茨血红蛋白（Hb Graz）。第二种变体与舍伍德森林血红蛋白或α2β2(104)(G6)精氨酸→苏氨酸相同；据信这可能是该异常血红蛋白的第二次观察报道。
