Rattner J B, Wang T
Department of Anatomy, Faculty of Medicine, University of Calgary, Alberta, Canada.
J Cell Sci. 1992 Dec;103 ( Pt 4):1039-45. doi: 10.1242/jcs.103.4.1039.
The potential for interphase centromeres to support kinetochore formation following premature chromosome condensation (PCC) has been investigated. We show that the centromere remains competent to initiate kinetochore formation throughout the cell cycle. PCC-kinetochores display a typical trilaminar morphology, associate with microtubules and show movement towards the centrosome. Indirect immunofluorescence studies illustrate that the centromere/kinetochore region of prematurely condensed chromosomes associates with proteins that are normally found within this region in both a cell cycle-dependent and an independent manner.
对间期着丝粒在染色体早熟凝集（PCC）后支持动粒形成的潜力进行了研究。我们发现，着丝粒在整个细胞周期中都保持着启动动粒形成的能力。PCC-动粒呈现出典型的三层形态，与微管相关联，并向中心体移动。间接免疫荧光研究表明，早熟凝集染色体的着丝粒/动粒区域以细胞周期依赖性和非依赖性方式与通常在该区域发现的蛋白质相关联。
