Bernat R L, Delannoy M R, Rothfield N F, Earnshaw W C
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cell. 1991 Sep 20;66(6):1229-38. doi: 10.1016/0092-8674(91)90045-z.
The relationship between the kinetochore and the centromeric heterochromatin that surrounds it is unknown. Anti-centromere autoantibodies (ACAs) that recognize antigens found in the heterochromatin beneath the kinetochore disrupt mitotic events when microinjected into human cells. We show here that ACAs interfere with two different stages of centromere assembly during interphase, resulting in abnormal kinetochore structures during mitosis. Antibody injection prior to late G2 results in the subsequent failure to assemble a trilaminar kinetochore. Such chromosomes bind microtubules but are incapable of movement. Antibody disruption of events during G2 produces unstable kinetochores that prevent the normal transition into anaphase. These experiments present a novel way to examine events in the pathway of kinetochore assembly that occur during interphase, at a time when this structure cannot be visualized directly.
着丝粒与其周围的着丝粒异染色质之间的关系尚不清楚。识别着丝粒下方异染色质中发现的抗原的抗着丝粒自身抗体（ACA），当显微注射到人类细胞中时会破坏有丝分裂事件。我们在此表明，ACA在间期干扰着丝粒组装的两个不同阶段，导致有丝分裂期间着丝粒结构异常。在G2晚期之前注射抗体导致随后无法组装三层着丝粒。这样的染色体能结合微管，但无法移动。在G2期期间抗体对事件的破坏产生不稳定的着丝粒，从而阻止正常过渡到后期。这些实验提供了一种新方法，可在间期检查着丝粒组装途径中发生的事件，而此时该结构无法直接可视化。
