Bauer G E, Balsamo J, Lilien J
Department of Biological Sciences, Clemson University, South Carolina 29634.
J Cell Sci. 1992 Dec;103 ( Pt 4):1235-41. doi: 10.1242/jcs.103.4.1235.
Rat pancreatic islet cells and RINm5F cells, an islet derived cell line, have at their cell surface an N-acetylgalactosaminylphosphotransferase (GalNAcPTase) similar to that found at the surface of chick neural retina cells and at the rat neuromuscular junction. On islet cells and RINm5F cells the GalNAcPTase is stably associated with cadherin cell-cell adhesion molecules. The effect of antibodies directed against the GalNAcPTase on homophilic, cadherin mediated adhesion was analyzed by measuring their effect on adhesion of islet and RINm5F cells to an immobilized anti-cadherin antibody. In this experimental paradigm anti-GalNAcPtase antibodies completely inhibit cadherin mediated adhesion. Furthermore, cadherin and GalNAcPTase co-distribute in islet and non-islet tissue. We conclude that pancreatic islet cell-cell adhesion is cadherin mediated and under the control of a tightly associated, cell surface GalNAcPTase.
大鼠胰岛细胞和RINm5F细胞（一种源自胰岛的细胞系）在其细胞表面具有一种N-乙酰半乳糖胺磷酸转移酶（GalNAcPTase），该酶类似于在鸡神经视网膜细胞表面和大鼠神经肌肉接头处发现的那种酶。在胰岛细胞和RINm5F细胞上，GalNAcPTase与钙黏蛋白细胞间黏附分子稳定相关。通过测量针对GalNAcPTase的抗体对胰岛细胞和RINm5F细胞与固定化抗钙黏蛋白抗体黏附的影响，分析了这些抗体对同源性、钙黏蛋白介导的黏附的作用。在这个实验范式中，抗GalNAcPtase抗体完全抑制钙黏蛋白介导的黏附。此外，钙黏蛋白和GalNAcPTase在胰岛和非胰岛组织中共分布。我们得出结论，胰腺胰岛细胞间黏附是由钙黏蛋白介导的，并受紧密相关的细胞表面GalNAcPTase的控制。
