Kodaka T, Toko T, Debari K, Hisamitsu H, Ohmori A, Kawata S
Department of Oral Anatomy, School of Dentistry, Showa University, Tokyo, Japan.
J Electron Microsc (Tokyo). 1992 Oct;41(5):381-6.
The environmental SEM (E-SEM) can be used unfixed biological samples under a low vacuum and wet condition. In this study, the fractured dentin of unfixed human teeth was treated with a 30% hydrogen peroxide solution (H2O2) for the examination of tooth-bleaching prior to the E-SEM and a conventional SEM. The peritubular matrix (PM) always showed a few cracks along the long axis of a dentinal tubule, and the ends of fine fibrils rose to the smoothly changed surface of the intertubular matrix (IM). The E-SEM with non-fixation and the conventional SEM following fixation indicated that the hydrogen peroxide solution easily permeated the PM and dissolved the non-fibrillar substance including the cracks of the PM by the constriction. In the IM, the solution may partially dissolve the organic parts within mineralized fibrils as well as non-fibrillar substance between the fibrils, although these remnants might precipitate again there.
环境扫描电子显微镜（E-SEM）可在低真空和潮湿条件下对未固定的生物样本进行观察。在本研究中，未固定的人类牙齿的断裂牙本质在进行E-SEM和传统扫描电子显微镜观察之前，先用30%的过氧化氢溶液（H2O2）处理以检查牙齿漂白情况。管周基质（PM）总是沿牙本质小管的长轴显示出一些裂纹，细纤维的末端升至管间基质（IM）平滑变化的表面。未固定的E-SEM和固定后的传统扫描电子显微镜显示，过氧化氢溶液很容易渗透到PM中，并通过收缩作用溶解包括PM裂纹在内的非纤维状物质。在IM中，该溶液可能会部分溶解矿化纤维内的有机部分以及纤维之间的非纤维状物质，尽管这些残余物可能会再次在那里沉淀。
