Pakarinen P, Huhtaniemi I
Department of Physiology, University of Turku, Finland.
J Endocrinol. 1992 Dec;135(3):507-15. doi: 10.1677/joe.0.1350507.
The responses of gonadotrophin gene expression, pituitary content and serum levels to castration alone and castration plus testosterone replacement (silicone elastomer implants) were compared in male rats at 10, 30, 60 and 90 days of age. Sham-operated animals served as controls. In addition, 30-day-old castrated rats were treated with dihydrotestosterone (DHT) and diethylstilboestrol (DES). When killed 7 days after castration, the increases in serum LH (six- to eightfold; P < 0.01) and FSH (two- to fourfold; P < 0.01) were similar at all ages studied. Likewise, testosterone reversed the effects of castration in a largely similar fashion at all ages. In contrast, great age-related differences were observed in the responses of gonadotrophin subunit mRNAs to the treatments. Castration increased the common alpha subunit mRNA two- to fourfold on days 10 and 30 (P < 0.01), sixfold on day 60 (P < 0.01), but not at all on day 90. Testosterone reversed the increases at all ages, but the levels were below those of controls only at 90 days (P < 0.01). The highest increases (sixfold; P < 0.01) of LH-beta mRNA were seen on days 10 and 60, the others being two- to threefold higher (P < 0.05-0.01). Testosterone reversed this effect at 60 days and suppressed LH-beta mRNA to below the control levels at other ages (P < 0.01). Castration had no effect on FSH-beta subunit mRNA at 30 and 90 days but a four- to fivefold increase was seen on days 10 and 60 (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
比较了10、30、60和90日龄雄性大鼠单独去势以及去势加睾酮替代（硅橡胶植入物）后促性腺激素基因表达、垂体含量和血清水平的反应。假手术动物作为对照。此外，对30日龄去势大鼠用双氢睾酮（DHT）和己烯雌酚（DES）进行处理。去势7天后处死动物时，在所研究的所有年龄组中，血清促黄体生成素（LH）升高6至8倍（P<0.01）和促卵泡生成素（FSH）升高2至4倍（P<0.01）的情况相似。同样，睾酮在所有年龄组中以大致相似的方式逆转了去势的影响。相比之下，观察到促性腺激素亚基mRNA对这些处理的反应存在很大的年龄相关差异。去势使10日龄和30日龄时共同的α亚基mRNA增加2至4倍（P<0.01），60日龄时增加6倍（P<0.01），但90日龄时无增加。睾酮在所有年龄组中都逆转了这种增加，但仅在90日龄时水平低于对照组（P<0.01）。LH-β mRNA在10日龄和60日龄时升高最高（6倍；P<0.01），其他年龄组升高2至3倍（P<0.05 - 0.01）。睾酮在60日龄时逆转了这种作用，并在其他年龄组将LH-β mRNA抑制到低于对照水平（P<0.01）。去势在30日龄和90日龄时对FSH-β亚基mRNA无影响，但在10日龄和60日龄时增加4至5倍（P<0.01）。（摘要截断于250字）
