Tamura T, Kitawaki J, Yamamoto T, Osawa Y, Kominami S, Takemori S, Okada H
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
J Endocrinol. 1992 Dec;135(3):589-95. doi: 10.1677/joe.0.1350589.
Immunohistochemical localization of 17 alpha-hydroxylase/C17-20 lyase (P-450(17 alpha,lyase)) and aromatase cytochrome P-450 (P-450arom) in normal human ovaries during the menstrual cycle was studied using specific polyclonal antibodies which were raised against corresponding enzymes. In the follicular phase of matured follicles, P-450(17 alpha,lyase) was localized in theca interna cells and P-450arom in granulosa cells. P-450(17 alpha,lyase) was expressed in theca interna cells before P-450arom was expressed in granulosa cells. The corpus luteum showed immunoreactivity to both enzymes and, after menstruation, immunoreactivity decreased gradually until it could not be detected in the corpus albicans. In corpus luteum graviditatis the immunoreactivity continued to be expressed strongly. In some atretic follicles, P-450(17 alpha,lyase) and/or P-450arom continued to be expressed. In the stromal layer, P-450(17 alpha,lyase was detected in secondary interstitial cells, which originated from the theca interna of atretic follicles, and P-450arom was detected in hilar cells. Immunoreactivity to both enzymes was also detected in oocytes of developing follicles. These results are consistent with the two cell theory in the human ovary. They also suggest that androgens and oestrogens are produced not only by follicles and corpora lutea but also by stroma and oocytes.
利用针对相应酶产生的特异性多克隆抗体，研究了月经周期中正常人类卵巢中17α-羟化酶/C17-20裂解酶（P-450(17α,裂解酶)）和芳香化酶细胞色素P-450（P-450arom）的免疫组织化学定位。在成熟卵泡的卵泡期，P-450(17α,裂解酶)定位于卵泡内膜细胞，P-450arom定位于颗粒细胞。P-450(17α,裂解酶)在卵泡内膜细胞中表达，随后P-450arom在颗粒细胞中表达。黄体对这两种酶均有免疫反应性，月经后，免疫反应性逐渐降低，直至在白体中无法检测到。在妊娠黄体中，免疫反应性持续强烈表达。在一些闭锁卵泡中，P-450(17α,裂解酶)和/或P-450arom持续表达。在基质层中，在起源于闭锁卵泡卵泡内膜的次级间质细胞中检测到P-450(17α,裂解酶)，在门细胞中检测到P-450arom。在发育卵泡的卵母细胞中也检测到对这两种酶的免疫反应性。这些结果与人卵巢的双细胞理论一致。它们还表明，雄激素和雌激素不仅由卵泡和黄体产生，也由基质和卵母细胞产生。
