Frankel E N, German J B, Davis P A
Department of Food Science and Technology, University of California, Davis 95616.
Lipids. 1992 Dec;27(12):1047-51. doi: 10.1007/BF02535586.
We previously described a rapid headspace gas chromatographic method for the determination of hexanal, an important decomposition product of n-6 polyunsaturated fatty acid (PUFA) peroxidation in rat liver samples and human red blood cell membranes. This method was applied to the measurement of Cu2+ catalyzed-oxidation of freshly prepared human low density lipoproteins (LDL) from 10 healthy adult volunteers. A twofold variation in oxidative susceptibility was found by this assay for hexanal and other volatiles. Hexanal values correlated significantly (P < 0.05) with total polyunsaturated fatty acid (PUFA), 18:2 and n-6 PUFA contents of LDL; but poorly with 20:4 and with vitamin E. Therefore, in addition to alpha-tocopherol, other endogenous antioxidants and factors may contribute to LDL's resistance to oxidation. This simple, rapid and sensitive method for oxidative susceptibility provides a useful component in the analysis of the prooxidant/antioxidant status of biological samples. The method is used routinely in our laboratories to determine specific peroxidation products of n-6 and n-3 PUFA.
我们之前描述了一种快速顶空气相色谱法，用于测定己醛，己醛是大鼠肝脏样本和人红细胞膜中n-6多不饱和脂肪酸（PUFA）过氧化的一种重要分解产物。该方法应用于测定来自10名健康成年志愿者的新鲜制备的人低密度脂蛋白（LDL）的铜离子催化氧化。通过该测定法发现，己醛和其他挥发性物质的氧化敏感性存在两倍的差异。己醛值与LDL中的总多不饱和脂肪酸（PUFA）、18:2和n-6 PUFA含量显著相关（P < 0.05）；但与20:4和维生素E的相关性较差。因此，除了α-生育酚外，其他内源性抗氧化剂和因素可能有助于LDL抵抗氧化。这种简单、快速且灵敏的氧化敏感性测定方法为分析生物样品的促氧化剂/抗氧化剂状态提供了一个有用的组成部分。该方法在我们实验室中常规用于测定n-6和n-3 PUFA的特定过氧化产物。
