金黄色葡萄球菌的噬菌体分型、血清学分型及酶学分型的比较研究

A comparative study of bacteriophage, serological and enzymic typing of Staphylococcus aureus.

作者信息

Tierno P M, Milstoc M

出版信息

Ann Clin Lab Sci. 1978 May-Jun;8(3):244-8.

Abstract

The presently accepted method for marking individual strains of Staphylococcus aureus for epidemiological investigation is bacteriophage typing. However, phage typing is not a stable marker and many strains cannot be successfully typed. Serological typing is not readily available and preparation of sera is difficult. The aminopeptidase profile method described by Krawezyk and Huber was used to mark strains of S. aureus. Profiles were constructed diagramming the percent hydrolysis of 22 beta-naphthylamide substrates by 15 isolates of S. aureus. The aminopeptidase profiles (APP) were thought to be more complete in marking individual strains of S. aureus when compared to bacteriophage and serological typing. Thusly, this method has applicability in the clinical laboratory for epidemiological investigation of S. aureus.

摘要

目前公认的用于标记金黄色葡萄球菌各个菌株以进行流行病学调查的方法是噬菌体分型。然而，噬菌体分型不是一个稳定的标记，许多菌株无法成功分型。血清学分型不易获得，且血清制备困难。Krawezyk和Huber描述的氨肽酶谱方法被用于标记金黄色葡萄球菌菌株。通过15株金黄色葡萄球菌对22种β-萘酰胺底物的水解百分比构建图谱。与噬菌体分型和血清学分型相比，氨肽酶谱（APP）被认为在标记金黄色葡萄球菌各个菌株方面更完整。因此，该方法在临床实验室中可用于金黄色葡萄球菌的流行病学调查。