通过氨肽酶谱区分表皮葡萄球菌生物型1和4的菌株

Differentiation of strains of Staphylococcus epidermidis biotypes 1 and 4 by aminopeptidase profiles.

作者信息

Tierno P M, Stotzky G

出版信息

Ann Clin Lab Sci. 1978 Mar-Apr;8(2):111-6.

Abstract

Seven different strains of Staphylococcus epidermidis and one strain of S. aureus were catagorized by their aminopeptidase activity. Eight different profiles were obtained: five profiles for S. epidermidis biotype 4 (each representing a different known serotype), two profiles for S. epidermidis biotype 1 (one for each strain used) and one for the strain of S. aureus used. Despite some similarities, distinct patterns of the hydrolysis of 22 different amino acid-beta-naphthylamides were evident for each individual strain. The evaluation of aminopeptidase profiles provides another means for distinguishing between strains of the same species and can, therefore, be used advantageously and in combination with serotyping as an epidemiological tool.

摘要

七种不同的表皮葡萄球菌菌株和一种金黄色葡萄球菌菌株通过它们的氨肽酶活性进行了分类。获得了八种不同的谱型：表皮葡萄球菌生物型4有五种谱型（每种代表一种不同的已知血清型），表皮葡萄球菌生物型1有两种谱型（每种用于实验的菌株各一种），以及一种用于实验的金黄色葡萄球菌菌株的谱型。尽管存在一些相似之处，但每种单独的菌株对22种不同氨基酸-β-萘酰胺的水解模式明显不同。氨肽酶谱型的评估为区分同一物种的菌株提供了另一种方法，因此，可以作为一种流行病学工具与血清分型结合使用，具有很大的优势。