[1-14C]乙酰肉碱的制备与纯化
A preparation and purification of [1-14C]acetylcarnitine.
作者信息
Roughan G, Browse J
机构信息
Horticulture Research Institute, Auckland, New Zealand.
出版信息
Anal Biochem. 1992 Nov 15;207(1):106-8. doi: 10.1016/0003-2697(92)90509-6.
PMID:1489081
Abstract
[1-14C]Acetylcarnitine was prepared from [1-14C]acetate and L-carnitine using acetyl-CoA synthetase and carnitine acetyltransferase. The product was purified by ion-exchange and thin-layer chromatography. Conversion of [1-14C]acetate to [1-14C]acetylcarnitine was better than 90% and overall recovery of the pure product was greater that 80%.
摘要
使用乙酰辅酶A合成酶和肉碱乙酰转移酶，由[1-¹⁴C]乙酸盐和L-肉碱制备[1-¹⁴C]乙酰肉碱。产物通过离子交换和薄层色谱法进行纯化。[1-¹⁴C]乙酸盐向[1-¹⁴C]乙酰肉碱的转化率高于90%，纯产物的总回收率大于80%。
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