Hasegawa T, Tanaka T, Yoshikai Y
Laboratory of Germfree Life, Nagoya University School of Medicine, Japan.
Int Immunol. 1992 Oct;4(10):1129-36. doi: 10.1093/intimm/4.10.1129.
We have previously reported that gamma delta T cells play important roles in protection during the early stage of infection with Listeria monocytogenes in mice. To generalize the protective roles of gamma delta T cells in listerial infection to different species, we examined the appearance of gamma delta T cells during infection with L. monocytogenes in Fisher F344 rats. The numbers of bacteria in the peritoneal cavity and liver increased to a maximum level on day 3 and then decreased to an undetectable level by day 10 after an intraperitoneal infection with a sublethal dose (1 x 10(8)) of viable L. monocytogenes in rats. CD3+ alpha beta- T cells in the peritoneal cavity and liver began to increase on day 3, reached a maximum level on day 6, and thereafter decreased gradually by day 10 after infection. Northern blot analysis confirmed that the CD3+ alpha beta- T cells expressed TCR delta and gamma gene messages. In vivo treatment with anti-TCR alpha beta mAb, which suppressed most of the alpha beta T cells in the periphery and impaired resistance during the late stage of listerial infection, did not affect the host defense by day 6 after infection. A significantly increased number of gamma delta T cells was detected in the peritoneal cavity of the TCR alpha beta-suppressed rats on day 6 after infection. These results suggest that the early appearing gamma delta T cells may contribute to the host defense at a relatively early stage during listeriosis in rats.
我们之前报道过，γδ T细胞在小鼠感染单核细胞增生李斯特菌的早期阶段发挥着重要的保护作用。为了将γδ T细胞在李斯特菌感染中的保护作用推广到不同物种，我们检测了Fisher F344大鼠感染单核细胞增生李斯特菌期间γδ T细胞的出现情况。大鼠经腹腔注射亚致死剂量（1×10⁸）的活单核细胞增生李斯特菌后，腹腔和肝脏中的细菌数量在第3天增加到最高水平，然后在第10天降至检测不到的水平。腹腔和肝脏中的CD3⁺αβ⁻ T细胞在感染后第3天开始增加，在第6天达到最高水平，此后在第10天逐渐减少。Northern印迹分析证实，CD3⁺αβ⁻ T细胞表达TCRδ和γ基因信息。在体内用抗TCRαβ单克隆抗体治疗，该抗体抑制了外周大部分αβ T细胞并损害了李斯特菌感染后期的抵抗力，但在感染后第6天并未影响宿主防御。在感染后第6天，在TCRαβ抑制的大鼠腹腔中检测到γδ T细胞数量显著增加。这些结果表明，早期出现的γδ T细胞可能在大鼠李斯特菌病的相对早期阶段对宿主防御有贡献。
