HOBERMAN H D, METZ C B, GRAFF J
J Gen Physiol. 1952 Mar;35(4):639-43. doi: 10.1085/jgp.35.4.639.
When fertilized and unfertilized eggs of Arbacia punctulata are suspended in heavy water, deuterium is incorporated into stable positions in the egg proteins. The rate of incorporation of the isotope is considerably greater in fertilized than in unfertilized eggs, and is accelerated at the time of formation of the blastula. The result of calculation of the maximum deuterium concentration which would be reached on complete turnover indicates that at least one out of every ten stably bound hydrogen atoms of the egg proteins is a deuterium atom. This has been interpreted as evidence that at the time of formation of the sea urchin blastula and in the period of development which follows, synthesis and breakdown are simultaneous processes leading to the redistribution of amino acids among the egg proteins.
当把海胆的受精卵和未受精卵悬浮在重水中时，氘会掺入到卵蛋白的稳定位置。同位素的掺入速率在受精卵中比未受精卵中要大得多，并且在囊胚形成时会加速。对完全周转时将达到的最大氘浓度的计算结果表明，卵蛋白中每十个稳定结合的氢原子中至少有一个是氘原子。这被解释为证据，表明在海胆囊胚形成时以及随后的发育阶段，合成和分解是同时进行的过程，导致氨基酸在卵蛋白之间重新分布。
