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J Biophys Biochem Cytol. 1960 Feb;7(1):21-6. doi: 10.1083/jcb.7.1.21.
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J Cell Biol. 1966 Jan;28(1):127-37. doi: 10.1083/jcb.28.1.127.
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本文引用的文献

1
The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.分裂细胞有丝分裂器的分离与生化特性分析
Proc Natl Acad Sci U S A. 1952 Sep;38(9):826-38. doi: 10.1073/pnas.38.9.826.
2
Experimental studies on amphibian oocyte nuclei. I. Investigation of the structure of the nuclear membrane by means of the electron microscope.两栖类卵母细胞核的实验研究。I. 利用电子显微镜对核膜结构的研究。
Proc R Soc Lond B Biol Sci. 1950 Oct 13;137(888):367-78. doi: 10.1098/rspb.1950.0047.
3
The ultrastructure of the nuclear membrane of the sea urchin oocyte as studied with the electron microscope.用电子显微镜研究海胆卵母细胞核膜的超微结构。
Exp Cell Res. 1955 Feb;8(1):147-58. doi: 10.1016/0014-4827(55)90051-3.
4
The isolation and preliminary characterization of a major soluble protein of the sea urchin egg.海胆卵一种主要可溶性蛋白的分离与初步鉴定
Exp Cell Res. 1959 Jan;16(1):59-69. doi: 10.1016/0014-4827(59)90195-8.
5
The fine-structure of the mitotic spindle in sea urchin eggs.海胆卵有丝分裂纺锤体的精细结构。
J Ultrastruct Res. 1958 Nov;2(1):55-72. doi: 10.1016/s0022-5320(58)90047-9.
6
The origin and fate of annulate lamellae in maturing sand dollar eggs.成熟海胆卵中环孔板的起源与命运。
J Biophys Biochem Cytol. 1959 Jan 25;5(1):117-22. doi: 10.1083/jcb.5.1.117.
7
Cytochemical localisations and ultrastructure in the fertilized unsegmented egg of Paracentrotus lividus.紫海胆未分裂受精卵中的细胞化学定位与超微结构
J Biophys Biochem Cytol. 1958 Sep 25;4(5):575-7. doi: 10.1083/jcb.4.5.575.
8
An electron microscope study of the nuclear envelope of amphibian oocytes.两栖类卵母细胞核膜的电子显微镜研究。
J Ultrastruct Res. 1958 Apr;1(3):201-22. doi: 10.1016/s0022-5320(58)80001-5.
9
Thin sections. II. A simple method for reducing compression artifacts.薄片。II. 一种减少压缩伪影的简单方法。
J Biophys Biochem Cytol. 1958 May 25;4(3):345-8. doi: 10.1083/jcb.4.3.345.
10
Electron microscopic and cytochemical studies on the cytoplasmic RNA in sea-urchin eggs.海胆卵细胞质RNA的电子显微镜和细胞化学研究。
Okajimas Folia Anat Jpn. 1958 Mar;30(6):369-87. doi: 10.2535/ofaj1936.30.6_369.

部分蛋白质提取对海胆(刺冠海胆)卵结构的影响。

The effect of partial protein extraction on the structure of the eggs of the sea urchin, Arbacia punctulata.

作者信息

KANE R E

出版信息

J Biophys Biochem Cytol. 1960 Feb;7(1):21-6. doi: 10.1083/jcb.7.1.21.

DOI:10.1083/jcb.7.1.21
PMID:14404287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2224858/
Abstract

The structure of the eggs of the sea urchin, Arbacia punctulata, has been investigated after the removal of one-half of the cellular protein. The procedure involves treatment of the eggs with 30 per cent ethanol at -10 degrees C. followed by extraction of the soluble proteins with water. The eggs remain intact, although all of the cytoplasmic matrix is removed. Most cell structures can still be identified, although only the membranes of most remain. The mitochondria lose all of their matrix but retain the inner membranes or cristae. The annulate lamellae appear unaffected by this extraction procedure, remaining intact and apparently undamaged. The nuclear envelope is also retained, although it often undergoes a curious disorganization, apparently as the result of the separation of its two layers. The significance of these observations with respect to the structure of the envelope is discussed.

摘要

在去除一半细胞蛋白质后,对海胆(刺冠海胆)的卵结构进行了研究。该程序包括在-10摄氏度下用30%乙醇处理卵,然后用水提取可溶性蛋白质。尽管所有细胞质基质都被去除,但卵仍保持完整。大多数细胞结构仍然可以识别,尽管大多数只剩下膜。线粒体失去了所有基质,但保留了内膜或嵴。环状片层似乎不受此提取程序的影响,保持完整且显然未受损。核膜也被保留下来,尽管它经常会发生奇怪的解体,显然是其两层分离的结果。讨论了这些观察结果对包膜结构的意义。