前脑啡肽原RNA在遭受社会剥夺应激的大鼠下丘脑内增加。
Preproenkephalin RNA increases in the hypothalamus of rats stressed by social deprivation.
作者信息
Iglesias T, Montero S, Otero M J, Parra L, Fuentes J A
机构信息
Department of Pharmacology, School of Medicine, Universidad Complutense, Madrid, Spain.
出版信息
Cell Mol Neurobiol. 1992 Dec;12(6):547-55. doi: 10.1007/BF00711234.
Abstract
- Pharmacological evidence indicates that stress induced by brief (14 to 20-day) social deprivation in the rat is associated with an activation of the central preproenkephalin (ENK) opioid system. This study examines the neurochemical evidence that substantiates such an activation. 2. Using a specific ENK complementary DNA probe, ENK RNA levels were measured by dot blot and Northern blot analyses in different brain areas of socially deprived rats. Immunoreactivity to met-enkephalin-derived peptides was also evaluated by radioimmunoassay in the same brain regions. 3. Brief social deprivation increased the levels of ENK RNA and enkephalin immunoreactivity in whole hypothalamus. 4. Our data suggest that this type of stress appears to be associated to an induction of ENK gene transcription in hypothalamus.
摘要
- 药理学证据表明，大鼠短暂（14至20天）社会剥夺所诱导的应激与中枢前脑啡肽原（ENK）阿片系统的激活有关。本研究检验了证实这种激活的神经化学证据。2. 使用特异性ENK互补DNA探针，通过斑点印迹和Northern印迹分析测量社会剥夺大鼠不同脑区的ENK RNA水平。还通过放射免疫测定法在相同脑区评估对甲硫氨酸脑啡肽衍生肽的免疫反应性。3. 短暂的社会剥夺增加了整个下丘脑的ENK RNA水平和脑啡肽免疫反应性。4. 我们的数据表明，这种类型的应激似乎与下丘脑ENK基因转录的诱导有关。
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