Macaione S, Di Giorgio R M, De Luca G
Ital J Biochem. 1976 Sep-Oct;25(5):367-81.
PL kinase activity has been determined in primary and secondary subcellular fractions of ox retina. Enzymic activity is predominantly located in the soluble fraction (S3). About 65% of the recovered PL kinase activity of crude mitochondria is released from synaptosomal fraction after hypoosmotic treatment. PL kinase activity in supernatant fraction (S3) does not exceed the enzyme activity measured in the homogenate, excluding the presence of a particulate-bound inhibitor to PL kinase in the homogenate. The possible physiological significance of PL kinase cellular compartmentation has been considered.
已测定牛视网膜初级和次级亚细胞组分中的磷脂激酶活性。酶活性主要位于可溶性组分（S3）中。经低渗处理后，粗线粒体中约65%回收的磷脂激酶活性从突触体组分中释放出来。上清液组分（S3）中的磷脂激酶活性不超过匀浆中测得的酶活性，这排除了匀浆中存在与颗粒结合的磷脂激酶抑制剂的可能性。已考虑了磷脂激酶细胞区室化的可能生理意义。
