Paolucci M, D'Antonio M, Pierantoni R
Dipartimento di Zoologia, Naples, Italy.
Gen Comp Endocrinol. 1992 Dec;88(3):335-40. doi: 10.1016/0016-6480(92)90228-c.
An androgen-binding activity has been identified in nuclear extracts of the testis of the frog, Rana esculenta. A single class of high affinity (Kd = 2.5 +/- 0.6 x 10(-9) M), low-capacity binding sites was found. The binding was specific for androgens; 17 beta-estradiol displaced [3H]testosterone with an ID50 of 0.1 microM. Cytosolic binding activity has a low affinity and a high capacity and lacks specificity. The seasonal fluctuations in binding capacity did not correlate with the androgen peaks in plasma and testes between February and June and in September; periods coinciding with the resumption of spermatogenesis and the development of spermatids, respectively. The present data strongly support androgenic control of intratesticular function in vertebrates generally.
在食用蛙（Rana esculenta）睾丸的核提取物中已鉴定出一种雄激素结合活性。发现了一类单一的高亲和力（Kd = 2.5 +/- 0.6 x 10(-9) M）、低容量结合位点。这种结合对雄激素具有特异性；17β-雌二醇以0.1 microM的半数抑制浓度（ID50）取代了[3H]睾酮。胞质结合活性具有低亲和力和高容量且缺乏特异性。在2月至6月以及9月期间，结合能力的季节性波动与血浆和睾丸中的雄激素峰值不相关；这两个时期分别与精子发生的恢复和精子细胞的发育相吻合。目前的数据有力地支持了一般脊椎动物睾丸内功能受雄激素控制的观点。
