Joutsjoki V V, Torkkeli T K
Research Laboratories, Alko Ltd., Helsinki, Finland.
FEMS Microbiol Lett. 1992 Dec 1;78(2-3):237-43. doi: 10.1016/0378-1097(92)90033-k.
The glucoamylase P gene of the fungus Hormoconis resinae has been cloned and sequenced from a genomic library. The gene consists of a 2153-bp protein coding region including three introns. The usual number of introns in cloned fungal glucoamylase genes has been four and in some cases five. Two of the glucoamylase P gene introns contain a sequence resembling the consensus sequence found near the 3' splice site in the introns of the fungus Trichoderma reesei cellobiohydrolase 1 (cbh1) gene. The H. resinae glucoamylase P gene, under its own promoter, was introduced into T. reesei, but no expression could be detected.
树脂枝孢菌的葡糖淀粉酶P基因已从基因组文库中克隆并测序。该基因由一个2153bp的蛋白质编码区组成，包括三个内含子。已克隆的真菌葡糖淀粉酶基因中内含子的通常数量为四个，在某些情况下为五个。葡糖淀粉酶P基因的两个内含子包含一个类似于在里氏木霉纤维二糖水解酶1（cbh1）基因内含子的3'剪接位点附近发现的共有序列的序列。树脂枝孢菌葡糖淀粉酶P基因在其自身启动子的控制下被导入里氏木霉，但未检测到表达。
