编码糖化酶的土曲霉gla1基因的分子克隆与转录分析
Molecular cloning and transcriptional analysis of the Aspergillus terreus gla1 gene encoding a glucoamylase.
作者信息
Ventura L, González-Candelas L, Pérez-González J A, Ramón D
机构信息
Departamento de Biotecnología, Consejo Superior de Investigaciones Científicas, Valencia, Spain.
出版信息
Appl Environ Microbiol. 1995 Jan;61(1):399-402. doi: 10.1128/aem.61.1.399-402.1995.
Abstract
The Aspergillus terreus gla1 gene, coding for a glucoamylase, has been cloned by heterologous hybridization. The gene is interrupted by four introns and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The expression of the gene is induced by starch and maltose and repressed by glucose.
摘要
编码葡糖淀粉酶的土曲霉gla1基因已通过异源杂交克隆出来。该基因被四个内含子中断，编码一种具有N端催化结构域和C端淀粉结合结构域的蛋白质。该基因的表达受淀粉和麦芽糖诱导，受葡萄糖抑制。
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Mol Microbiol. 1993 Mar;7(6):847-57. doi: 10.1111/j.1365-2958.1993.tb01175.x.
2
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3
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Curr Genet. 1993 Sep;24(3):205-11. doi: 10.1007/BF00351793.
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Biotechnology (N Y). 1993 Jul;11(7):815-8. doi: 10.1038/nbt0793-815.
5
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8
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9
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