Wang P M, Lai-Fook S J
Center for Biomedical Engineering, University of Kentucky, Lexington 40506.
J Appl Physiol (1985). 1992 Dec;73(6):2314-9. doi: 10.1152/jappl.1992.73.6.2314.
In anesthetized, paralyzed, supine rabbits (3-4 kg) during apnea, we injected fluorescent dye or fluorescent microspheres (2 or 6 microns diam) into the dependent pleural space and observed the arrival and movement of the dye or microspheres at superior regions. Injection was through a rib capsule located in the dependent right chest. The dye or microspheres were observed through a pleural window overlying a lobar margin. The vertical distance between the capsule and window was 3-4 cm. The movement of the dye or microspheres was recorded via a fluorescence videomicroscope, and the signals were analyzed for dye transit time and microsphere velocity. The transit time of the dye to traverse the height of the pleural space was calculated from the light intensity vs. time curve. Transit time during apnea averaged 6.0 +/- 3.4 (SD) min (n = 4). Transit time measured after the onset of mechanical ventilation was < 1 min. The direction and speed of a microsphere moving in the relatively thick pleural space adjacent to the lobar margin depended on its distance from the lobar margin. Microspheres moved upward in the pleural space that was in proximity to the lobar margin but downward at farther distances from the lobar margin. Pleural liquid recirculation occurs via the pleural space adjacent to lobar margins.
在呼吸暂停期间，对麻醉、麻痹且仰卧的3 - 4千克家兔，我们将荧光染料或荧光微球（直径2或6微米）注入下垂侧胸膜腔，并观察染料或微球到达上侧区域及在该区域的移动情况。注射通过位于右侧下垂胸部的肋膜进行。通过覆盖叶边缘的胸膜窗口观察染料或微球。注射部位与观察窗口之间的垂直距离为3 - 4厘米。通过荧光视频显微镜记录染料或微球的移动情况，并分析信号以确定染料通过时间和微球速度。染料穿过胸膜腔高度的通过时间根据光强度与时间曲线计算得出。呼吸暂停期间的通过时间平均为6.0 +/- 3.4（标准差）分钟（n = 4）。机械通气开始后的通过时间小于1分钟。在与叶边缘相邻的相对较厚的胸膜腔内移动的微球的方向和速度取决于其与叶边缘的距离。微球在靠近叶边缘的胸膜腔内向上移动，但在离叶边缘较远的地方向下移动。胸膜液体通过与叶边缘相邻的胸膜腔进行再循环。
